Angelova and Dimitrov

The so-called giant vesicles owe their name to the fact that they can reach up to 100 (xm in diameter. Such over-dimensioned structures can be often observed as byproducts in the normal preparation of vesicles, but they can be obtained by specific methods, for example by that dubbed electroformation (Angelova and Dimitrov, 1988). 

A totally different approach is described in this section here enzymatic reactions are performed in giant vesicles (GVs) with diameters of 30 to 100 pm. The GVs are formed by a method described by Angelova and Dimitrov,called the electroformation method. This method is singular in the sense that the formed GVs remain in contact with the platinum electrode (the site where they are formed), a fact that is crucial for further experimentation. Of course, these GVs cannot be considered... 

The method used in this work is the so-called electroformation method, originally developed by Angelova and Dimitrov [9], This method is of particular interest because it allows the preparation of giant vesicles with diameters around 100 pm, which can easily be used for microinjection experiments. The vesicles grow on a platinum wire under the influence of an alternating or direct electric field. 

Angelova, A Woinova, S. and Dimitrov, D., Differentialthermoanalytis-che Untersuchungen zur Effektivitat einiger Stabilisatoren bei PET, Angew. Makromol. Chem., 64, 75 (1977). 

Angelova, M. I. and Dimitrov, D. S. (1988). A mechanism of liposome electro-formation. Progr. Colloid Polymer. ScL, 76, 59-67. 

Dimitrov, D. S and Angelova, M. I., Lipid swelling and liposome formation mediated by electric fields, Bioelectrochem. Bioenerg., 19, 323-336 (1988). 

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