Analytical HPLC temperature pressure effect

The effects of sample preparation variability on assay variability are well known and should be considered when acceptable variations within the analytical method are set in place. Pipetting errors, sample collection errors, time, and temperature of sample preparation may all contribute to slight differences in the amount of analyte extracted or prepared within a given sample. Additionally, HPLC instrumentation may also exhibit injector or flow rate variability leading to differences in retention times and peak responses. Column aging and buildup of lipids and proteins within the HPLC components may ultimately cause pressure fluctuations and mechanical problems if the instrument is not properly maintained. 

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