Analyte bisphosphonates

Lovdahl, M. J. and Pietrzyk, D. J., Anion-exchange separation and determination of bisphosphonates and related analytes by post-column indirect fluorescence detection,. Chromatogr A, 850, 143, 1999. 

Derivatization is another form of sample preparation. It is utilized for the analysis of labile analytes or to enhance retention or detection with a preferred type of detector. Derivatization can be performed to enhance detection by UV/Vis, fluorescence, or electrochemical detection. Consideration must be given to the stability of the derivatize to solvolysis and thermal degradation. In our labs alendronate, a bisphosphonate with a primary amine functionality, was derivatized with FMOC to enhance detection by UV/Vis as well as to increase retention in RPLC mode [19]. An acylchloride was derivatized with... 

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