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Analysis of the GPI Anchor

Thus mass spectrometry showed the GPI was heterogeneous with at least five separate species and its composition was consistent with known GPI structures but with sialic acid as a novel component. The heterogeneity was confirmed by various separative techniques including capillary electrophoresis, Dionex high performance anion exchange chromatography (HPAEC), and reverse phase HPLC at pH 7. Cleavage [Pg.41]

A further difference between oligosaccharides and proteins arises from the presence of alternadve sites for linking individual sugars together. The glycosidic bond from carbon-1 of the nonreducing residue in a disaccha- [Pg.43]


Wang, Q., Fujioka, H., and Nussenzweig, V. (2005b). Mutational analysis of the GPI-anchor addition sequence from the circumsporozoite protein of Plasmodium. Cell Microbiol. 7, 1616-1626. [Pg.390]

Scheme 12.6 Retrosynthetic analysis of the GPI anchor from S. cerevisiae, by the Schmidt group. Small Roman numerals indicate the order of assembly. Scheme 12.6 Retrosynthetic analysis of the GPI anchor from S. cerevisiae, by the Schmidt group. Small Roman numerals indicate the order of assembly.

See other pages where Analysis of the GPI Anchor is mentioned: [Pg.29]    [Pg.39]   


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