Analysis of Procyanidins and Proanthocyanidins in Wine

Sample preparation by reverse phase chromatography is performed using a C18 (e.g. 20-mL) SPE cartridge a volume of 30 mL of 

Fractions 1-4 can be analyzed by ESI-direct infusion recording mass spectra in negative ionization mode. Due to the higher complexity of fractions 8-10, for these samples direct ESI-MS analysis does not provide useful information, and for their characterization LC/ESI-MS and MSn analyses have to be performed. Compounds identified are reported in Table 3.8 with the analytical conditions used. 

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