Analysis of l-Octen-3-one

TABLE 8.2. Analytical Conditions for GC/MS-IT Analysis of l-Octen-3-one in Wine2 

MS/MS acquisition conditions Acquisition interval (min) Retention time (min) Recorded mass range Quantification masses 

100% Dimethyl polysiloxane fused silica capillary (60m x 0.25 mm i.d. 1.00- xm film thickness) 

This compound is responsible for a fungal must taint reported in the wine cork industry. Characterized by unpleasant, musty, moldy odor, and a sensory threshold in white wine of 2.1 ng/L, this compound has been assessed as the second cause of cork taint (Simpson et al., 2004). Bacteria capable of producing 2-methoxy-3,5-dimethylpyrazine could be present in areas where the cork is processed or stored. 

For wine analysis, 3-g sodium tetraborate is added to 100 mL of sample and liquid-liquid extraction is performed with pentane (2 x 10mL). To purify the extract, the organic solution is washed with water (5mL), then extracted with cold sulfuric acid 1M (2 x 10mL). The acid 

---