Analysis of Intersection Points

Intersection points in the double reciprocal plots of i/vo versus A or fvo versus l/B, in bisubstrate reactions, provide a criterion for an initial estimate of the reaction mechanism (Rudolph Fromm, 1979). The choice of a mechanism listed in Table 4 could be made on the basis of the evaluation of the points of intersection in the double reciprocal plots in the forward and reverse directions. 

The Ordered and TheoreU-Chance mechanisms have certain constraints placed on their intersection points, but the Rapid Equilibrium Random mechanism has none, since only the equilibrium constant relates the forward and reverse reactions. 

N indicates that intersection on that position fs not possible for that particular mechanism. 

Intersection points in primary and secondary double reciprocal plots are very characteristic, with coordinates that are often simple expressions composed of kinetic constants. It is often possible to obtain the initial estimate of kinetic constants already from the inspection of intersection points in the primary plots. A simple method for the calculation of coordinates of intersection points was described in Chapter 9 (Section 9.2). 

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