Analysis of Anthocyanins and Derivatives

Time (min) formic acid/water 10 90 % formic acid/methanol/water 10 50 40 (v/v/v) % 

Concentration of the single anthocyanin is expressed in mg/Kg grape of malvidin-3-glucoside, the compound used as external standard to 

In general, by HPLC and UV-Vis detection it is possible to analyze some anthocyanin derivatives in wine, such as vitisins, several pyranoantho-cyanins and flavanol-ethyl-anthocyanin derivatives. On the other hand, due to the absence of standards commercially available, identification and quantification of these compounds is difficult. 

Time (min) solvent A aqueous H3P04 1.5% (w/w) % solvent B solvent A/acetonitrile 20 80 (v/v) % 

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Sample Preparation for Analysis of Anthocyanins and Derivatives in Wines... 

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