Analysis of a Diet Soft Drink by HPLC

In this experiment, high-performance liquid chromatography (HPLC) will be used to identify the artificial addihves presenf in a sample of commercial dief soff drink. The experiment uses HPLC as an analytical tool for the separation and identification of the additive substances. The method uses a reversed-phase column and eluent system, with isochratic elution. Detection is accomplished by measuring the absorbance of ultraviolet radiation at 254 nm by the solution as it is eluted from the column. The mobile phase that will be used is a mixture of 80% 1 M acetic acid and 20% acetonitrile, buffered to pH 4.2. [Pg.450]

Diet soft drinks contain many chemical additives, including several substances that can be used as artificial sweeteners. Among these additives are the four substances that we will be detecting in this experiment caffeine, saccharine, benzoic acid, and aspartame. The structures of these compounds are shown here. [Pg.450]

You will identify each compound in a sample of diet soft drink by its retention time on the HPLC column. You will be provided with data for a reference mixfure of each substance in a test mixture in order to compare retention times in your test sample with a set of sfandards. [Pg.450]

New Technique 21 High-Performance Liquid Chromatography (HPLC) 450 [Pg.450]

The instructor will provide specific instruction in the operation of the particular HPLC instrument being used in your laboratory. The instructions that follow indicate the general procedure. [Pg.451]

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