An Introduction to UHPLC

The efficiency of a chromatographic separation can be described by the height equivalent of a theoretical plate (H), where lower values of H correspond to more efficient separations. The Van Deemter equation describes the relationship between H and mobile phase flow velocity (u) as the sum of three major terms, A, B, and C, each of which represents a different contribution to band broadening in a chromatographic column. 

000 for isocratic reversed-phase separations and peak capacities over 500 using gradient elution (Jerkovich et al., 2003). 

2 UHPLC for LC x LC High Speed Versus High Peak Capacity 

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