An enzyme-linked MIP sorbent assay

Competitive radioassays indicated a slight preference for the template over CPOAc or 2,4-DB (see Fig. 20.14), giving cross-reactivities of 25 and 10% cf. 24 and 95% Table 20.1). 

Having established the selectivity of the microspheres for 2,4-D, the template was then conjugated to tobacco peroxidase (TOP). Briefly, TOP was activated using NaI04 then coupled with diaminopropane. 2,4-D was converted to its N-hydroxysuccinimide ester and allowed to react with the derivatised TOP. After purification, the final 2,4-D/TOP ratio was estimated to be 8/1. 

---