Amylo-1,6-D-glucosidases

A glycogen-debranching enzyme (amylo-1,6-D-glucosidase) from dogfish muscle has been purified by a procedure that takes advantage of its adsorption onto... 

Amylo-1,6-D-glucosidase Arabinanase Arylsulphatase 4-L-Aspartyl-P-D-glucosylamine amidohydrolase Bromelain (juice) Bromelain (stem) Carbohydrate isomerases Carbohydrate transferases Carboxypeptidases A—C Carboxypeptidase Y Cellobiase... 

Amylo-1 —> 6-glucosidase obtained by Cori and Larner218 from rabbit muscles, and R-enzyme isolated by Hobson, Whelan and Peat219 from potatoes and broad beans, are typical debranching enzymes, which will hydrolyze the 6 — 1-a-D-glucosidic linkage rather than the normal 4 —> 1-a-D linkage. These enzymes will therefore be particularly important in determinations of the fine structure of amylopectin, if they can be sufficiently well purified. 

Amylo-(1 6)-glucosidase is the a-D-(l— 6)-debranching enzyme found in animal skeletal muscle, where it debranches glycogen. Methods of preparation have been described, but there is no knowledge regarding its structure or physical properties. 

Fig. 6.—Interconversion of Glycogen and n-Glucose by way of Glycogen Cycle. (Key (1), hexokinase-adenosine 5-triphospliate (2), phosphoglucomutaae (3),UDPG-pyrophoaphorylase (4), glycogen-UDPglucosyl transferase and branching enzyme (5), phosphorylase and amylo-l,6-glucosidase (6), D-glucose 6-phosphatase.)...

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