Amplification fragment length

Marsh, T. L. 1999. Terminal restriction fragment length polymorphism (T-RFLP) An emerging method for characterizing diversity among homologous populations of amplification products. Current Opinion in Microbiology 2 323-327. 

Amplified fragment length polymorphism (AFLP), developed by Vos et al. [453], is also a new technique for DNA fingerprinting. This technique is based on the selective amplification of restriction fragments (SARE) by PCR from a digest of total genomic DNA. 

Leuders, T., and Friedrich, M. W. (2003). Evaluation of PGR amplification bias by terminal restriction fragment length polymorphism analysis of smaU-subunit rma and mcra genes by using defined template mixtures of methanogenic pure cultures and soil DNA extracts. Appl. Environ. Microbiol. 69, 320-326. 

A recent study performed by Stroh et al. ° compared the performance of MALDI-TOF MS with restriction fragment length polymorphism (RFLP) and fluorescence polarization (FP). The study involved the analysis of known mutations of the IL-1(3 gene. The procedure involved amplification of patient DNA samples using standard PCR techniques followed by a primer extension step where a separate post-PCR primer is hybridized directly adjacent to the SNP site. The resulting MALDI-TOF MS data provided a direct... 

The answer is a. (Murray, pp 488-504. Scriver, pp 3-45. Sack, pp 41-45. Wilson, pp 151-186.) Restriction fragment length polymorphisms (RFLPs) arising from variable numbers of tandem repeats (VNTRs) are the basis of the DNA fingerprinting technique. The process is (1) isolation of DNA from parent/child or forensic specimens using blood, skin, or semen (2) PCR amplification and radioactive labeling of DNA from... 

Plant DNA analysis has been increasing over the past couple of years because of its ability to pinpoint the origin of drug-related plant material [ 101,102]. Plant STRs are not yet well characterized however, other molecular methods can be combined with capillary gel electrophoresis to establish the identity of plant species [103]. These techniques include amplified fragment length polymorphism (AFLP), random amplification of polymorphic DNA (RAPD), and other similar random primer annealing techniques used for mutation detection. 

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