Ampholytes synthetic carrier

Isoelectric Focusing (lEF) using Synthetic Carrier Ampholytes... 

Recently, alternative procedures have been investigated in an attempt to overcome the problems associated with the use of synthetic carrier ampholytes. We have assessed buffer lEF using the 47 component mixture, PolySep 47 (, ),for the first dimension of... 

It should be noted that protein loading tolerances are also dependent upon the pH range and the particular manufacturer of the synthetic carrier ampholytes. For example, more than twice the protein that can be applied to a pH 5-7 ampholyte (LKB) gel can be applied to a pH 4 5-5.5 Servalyte gel without any protein band distortion. While a gel containing pH 4.5-5.4 Pharmalytes can only handle 70% of the protein of the Servalyte gel (unpublished data). 

Carrier ampholytes Synthetic amphoteric compounds containing acidic and basic functions which migrate under the influence of an electric field to form a stable pH gradient in isoelectric focusing. The composition of the carrier ampholyte mixture defines the extent and shape of the pH gradient. 

Mixtures of synthetic molecules called carrier ampholytes are used to establish pH gradients under the influence of applied voltages.1 This is the most common and perhaps simplest means for gradient formation. It is the method of choice for most IEF runs. 

The first practical IEF experiments were carried out with the use of synthetic molecules, called carrier ampholytes, to generate the pH gradients.1,26 Carrier ampholytes are amphoteric electrolytes that carry both current and buffering capacity. Much of the early theoretical activity in electrofocusing dealt with the properties required of carrier ampholytes and is more or less irrelevant to a current discussion.1,3,9 Different varieties of... 

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