Aminopyrrolidine carboxylic acid

Ethyl 7-(3-aminopyrrolidin-1 -yl)-4-oxo-1 -(thiazol-2-yl)-1,4-dihydro-1,8-naphthyri-dine-3-carboxylate (2, R = Et) gave the corresponding 3-carboxylic acid (2, R = H) (10% HC1, reflux 89%).166... 

Cyclization of S-aminopyrrolidine induced from S(— )-proline and the iminoether of carboxylic acid were used to produce an iminazole... 

P-Pyrazol-l-yl-L-alanine (2-61), derived from pyrazole, occurs in some plants of the Cucurhitaceae family. It was first isolated from the seeds of watermelon Citrullus lanatus), where it represents approximately 0.1% of dry matter. It also occurs in the seeds of many other melons, squashes and gourds, together with other heterocyclic amino acids such as 3-aminopyrrolidine-3-carboxylic acid (2-52). 

Sample preparation Mix 100 jaL plasma, 500 p,L water, 560 p.L 100 mM pH 9.3 borate buffer, 10 p.L 100 p,M IS in water, and 420 p,L 762 (xg/mL DBD-F in MeCN, let stand at room temperature for 30 min (to derivatize thiol in metabolites and IS), evaporate the MeCN under reduced pressure, add 2 mL ethyl acetate to the remaining solution, mix vigorously, centrifuge at 3000 rpm for 2 min. Wash the aqueous layer three more times with ethyl acetate. Adjust the pH of the aqueous layer to 1-2 with ca. 1.5 mL 100 mM HCl, extract three times with 2 mL portions of ethyl acetate. Combine the organic layers and evaporate them to dr5Uiess under reduced pressure, reconstitute the residue with 100 xL MeCN. Remove a 50 p.L aliquot, add 5 p.L DPP A, add 45 xL 17.8 mg/mLi -(—)-DBD-Apy in MeCN, let stand at room temperature for 2 h (to derivatize carboxylic acid), inject an aliquot. (DBD-F is 4-()V,IV-dimethylaminosulfonyl)-7-fluoro-2,l,3-benzoxadiazole. i -(—)-DBD-Apy is i -(—)-4-( V,IV-dimethylaminosulfonyl)-7-(3-aminopyrrolidin-l-yl)-2,l,3-benzoxadiazole. DPPA is diphenyl phosphoryl azide. They are all available from Tokyo Kasei or TCI America (www.tciamerica.com).)... 

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