4-Aminoantipyrine hydrogen peroxide determination

Tamaoku and colleagues presented an efficient enzymatic photometric determination of hydrogen peroxide ffiat is essentially a color reaction resulting from the oxidative condensation of A/-ethyl-A/-(2-hydroxy-3-sulfopropyl)aniline derivatives wiffi 4-aminoantipyrine in the presence of hydrogen peroxide and peroxidase (82CPB2492). A similar calorimetric detection of hydrogen peroxide has been patented (83GEP3301470). 

Phenols react with 4-aminoantipyrine to give in-dophenols in the presence of hydrogen peroxide and peroxidase under weak alkaline conditions. The resultant indophenols are determined by absorptiometry. Potassium hexacynoferrate (III) can also be used as an oxidant. As the indophenols have electrochemical activity, this reaction is utilized for precolumn derivatization for LC-ECD. 

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