Amino oxidase trigonopsis variabilis

L-methionine DL-methionine + Asp D-amino acid oxidase + transaminase Trigonopsis variabilis ... 

Chemical synthesis and isolation of 2-keto-6-hydroxyhexanoic acid required several steps. In a second, more convenient process (Fig. 2), the ketoacid was prepared by treatment of racemic 6-hydroxynorleucine [produced by hydrolysis of 5-(4-hydroxybutyl)hydantoin (3)] with D-amino acid oxidase and catalase. After the e.e. of the remaining L-6-hydroxynorleucine had risen to >99%, the reductive animation procedure was used to convert the mixture containing 2-keto-6-hydroxyhexanoic acid and L-6-hydroxynorleucine entirely to L-6-hy-droxynorleucine with yields of 91-97% and e.e. of >98%. Sigma porcine kidney D-amino acid oxidase and beef liver catalase or Trigonopsis variabilis whole cells (source of oxidase and catalase) were used successfully for this transformation [22],... 

Fig. 5. Correlation between heat response and reaction rate of cephalosporin C transformation by immobilized D-amino acid oxidase of Trigonopsis variabilis. Enzyme immobilization techniques entrapment in polyacrylamide gel ( ), cells cross-linked with glutaraldehyde ( ), cells entrapped in polyacrylamide gel (a) [28]...

This approach was used in the study of the properties of D-amino acid oxidase isolated or fixed in cells of Trigonopsis variabilis and entrapped in calcium pectate or polyacrylamide gel [28]. The approach of a differential reactor (low enzyme activity in the packed bed) was applied. The experimental thermometric data, ATr, were transformed to reaction rates, vobs, according to Eq. (21), whereas parameter a was determined by the calibration shown in Fig. 5. The data were described by the equation... 

The operational stability of Trigonopsis variabilis cells with D-amino acid oxidase activity, entrapped in standard and hardened ionotropic gels, was also investigated by means of the FMC [39]. The activity of the biocatalyst packed in the FMC column was continuously monitored by the FMC signal measurement... 

Fig. 13. Operational stability of D-amino acid oxidase fixed in cells of Trigonopsis variabilis CCY 15-1-3 entrapped in standard (A) and hardened (a) calcium pectate gel and standard (O) and hardened calcium alginate gel ( ). The relative activity was monitored by continuous processing, with the substrate (cephalosporin C) solution in the flow microcalorimeter [39]...

Oxidative Deamination Catalyzed by Immobilized D-Amino Acid Oxidase from Trigonopsis variabilis (E.C. 1.4.3.3) [40 421... 

D-Amino Acid Oxidase, carrier-fixed Origin Trigonopsis variabilis... 

L-6-hydroxynorleucine with yields of 91 to 97% and ee > 99%. Sigma porcine kidney D-amino acid oxidase and beef liver catalase or Trigonopsis variabilis whole cells (source of oxidase and catalase) were used successfully for this transformation. 

L-methionine DL-methionine + Asp a-keto-7-methylthiobutyric acid + NH4 + formic acid D-amino acid oxidase + transaminase Phe dehydrogenase + formate Trigonopsis variabilis Sporosarcina ureae 195... 

S)-Amino-3-[3- 6-(2-methylphenyl) pyridyl]-propionic add 82a was prepared by an enzymatic deracemization process using a combination of two enzymes (P)-amino acid oxidase from Trigonopsis variabilis cloned and expressed in E. coli and an (S)-aminotransferase from Sporosarcina ureae, also cloned and expressed in E. coli [147]. Racemic amino acid 82 was used as a substrate and (S)-aspartate was used as amino donor. An (S)-aminotransferase was also purified from a soil organism identified as Burkholderia sp. and cloned and expressed in E. coli and used in this process [147]. This process was scaled up to 70 L scale. 

A one-pot chemoenzymatic synthesis of 3 -functionalized cephalosporin C or cepha-zolin involving three consecutive biotransformations has been reported [16]. The enzymatic deacylation of cephalosporin C in one batch was performed by the use of D-amino acid oxidase (DAO) from Trigonopsis variabilis, glutaryl acylase (GA) from Acetobacter sp., and a continuous flow of oxygen. DAO catalyzed the oxidative deamination of the a-... 

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