2- Amino-2-deoxynucleosides synthesis

A novel solid phase method for the synthesis of 2 -amino-2 -deoxynucleoside 5 -triphosphates has been developed in which the 3 -azidonucleoside precursors are first linked to support-bound triphenylphosphine as their phosphinimines 116. Following conversion into the triphosphate, the desired 2 -amino-2 -deoxy-nucleoside 5 -triphosphates are released from the support by treatment with ammonia in the Staudinger reaction. ... 

Several 2 -fluoro- and 2 -amino-2 -deoxynucleosides (99) have been characterised as good T7 RNA polymerase substrates and thus permit the enzymatic synthesis of modified RNA polymers by transcription. ... 

Other references to nucleoside antibiotics, including microbial synthesis of 2 -amino-2 -deoxy-nucleosides and Ara-A, the synthesis of oc-2 -deoxynucleosides, and the synthesis of radio-labelled... 

Amino-2 -deoxynucleosides 190 involving all the main nucleobases have been prepared by Mitsunobu reactions involving iV-hydroxyphthalimide the products are of relevance in the synthesis of antisense oligonucleotides (see Vol.26, p.242).2 ... 

Sinha, N. D, Biernat, J., McManus, J., and Koster, H (1984) Polymer support oligonucleotide synthesis XVIII, use of P-cyanoethyl-lV,V-dialkyl-amino-W-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product Nucl Acids Res 12,4539-4557. 

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