5- Amino-2-bromophenol

Bromophenol blue (3.0...4.6) aliphatic carboxylic acids [225 — 228] malonic and lactic acids [229] palmitic and lactic acids [230] malonic, glycolic, malic, citric, tartaric, ketoglutaric, galacturonic and oxalic acids [196] dicarboxylic acids, succinic acid [231] indoleacetic acid, trichloroacetic acid [232] palmitic acid, palmityl- and stearyllactic acid [223] benzoic, sorbic and salicylic acid [234] metabolites of ascorbic acid [235] chloropropionic acid [236] oligogalacturonic acids [237] amino acids, hydrocarbons, mono-, di- and triglycerides [238] xylobiose, xylose, glucose and derivatives [239] sugar alcohols [91] toxaphene [240]... 

Phenyl ureas, carbamates, organochlorine insecticides, 4-bromophenol, captan, bromo- and chloroanilines, methylanilines, bromo- and chloro-nitrobenzenes, diphenyl amino folpet, hexachlorobenzene, naphthalene, phenol, simazine... 

Do not stain the spots too strongly because if the the amount of absorbed bromophenol blue is too high, some of this dye could be incorporated in the peptide. In that case removal of the color is almost impossible and could affect the detection. Because bromophenol blue has a blue color in a basic environment, e.g., primary amino functions, a difference in the intensity of the color of the spots is normal due to different acidity of the coupled amino acids. 

Alizarin Alizarin Red S Alizarin Yellow R Benzopurpurine 4B 4,4 -Bis(2-amino-l-naphthylazo)-2,2 -stilbenedisulfonic acid 4,4 -Bis(4-amino-l-naphthylazo)-2,2 -stilbenedisulfonic acid Brilliant Yellow Bromocresol Green Bromocresol Purple Bromophenol Blue Bromothymol Blue Chlorophenol Red Clayton Yellow Congo Red o-Cresolphthalein Cresol Red Crystal Violet Curcumin (Turmaric) p-(2,4-Dihydroxyphenylazo) benzenesulfonic acid, sodium salt... 

Free amino functions on the spots can be stained with bromophenol blue (26) prior to the coupling reactions. This allows visual monitoring of the performance of all the synthesis steps. Thus, a standard membrane for SPOT-... 

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