Amino add dehydrogenase

T. Ohshima and K. Soda, Biotechnological aspects of amino add dehydrogenases,... 

Vedha-Peters, K., Gtmawardana, M., Rozzell, J.D., and Novick, S.J. (2006) Creation of a broad-range and highly stereoselective n-amino add dehydrogenase for the one-step synthesis of D-aminoacids./. Am. Chem. Soc., 128, 10923-10929. 

Like amine oxidases, one can also combine amino add dehydrogenases with in situ chemical reduction, a transaminase, or an amino add dehydrogenase to effect dynamic kinetic resolutions of amino adds. Details of these more complex processes are desaibed in the appropriate later chapters. 

In another alternate enzymatic process, racemic amino add 82 was first treated with (R)-amino acid oxidase for 4 h to convert 82 to a mixture of (S)-amino add 82a and keto add 83. Subsequently, an amino add dehydrogenase NADP and glucose dehydrogenase were added to the same reaction mixture to convert 83 to (S)-amino acid 82a, providing this product in 54% isolated yield and 99% ee. 

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