Amine or Hydrazide Molecules by Carbohydrates and Glycans

Modification of Amine or Hydrazide Molecules by Carbohydrates and Glycans 

Ring opening with reduced secondary amine linkage to C1 

Ring opening with reduced hydrazone linkage to C1 

Glycosylhydrazide formation with intact GIcNAc ring structure 

Add to the glycan solution the molecule to be labeled containing an available amine, hydrazine, or hydrazide group. For small molecule derivatization, the final concentration of the nucleophile in the glycan solution should be about 0.3 M to result in maximal efficiency of labeling. For protein modification, an aqueous reaction buffer should be used, and the protein should be as concentrated as possible. 

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