Alternative immunoassay methods with potential application in sensor development

5 Alternative immunoassay methods with potential application in sensor development [Pg.166]

Other methods with potential application in sensor development include certain enzyme immunoassays and sol-particle immunoassay. The enzyme methods that are potentially useful are those where the detection of the enzyme is carried out with a substrate that produces colour that may be determined colorimetrically, or a substrate that produces fluorescence. [Pg.166]

The method of sol-particle immunoassay uses colloidal metal particles as the label. Typically these labels have been colloidal gold and colloidal silver. Detection in this type of assay is carried out visually, colorimetrically or by atomic absorption spectroscopy with electrothermal atomization. The colorimetric methods obviously offer potential in sensor development and the detection limits in the pmol 1 range are useful. [Pg.166]

Although this section has discussed immunoassay methods with potential for the development of sensors based on fibre optics, electrochemical immunoassay methods (10) may also find application in sensors development. Of particular interest is the use of piezoelectric crystals in immunoassay (11). [Pg.166]

Albertini and R. Ekins, in Monoclonal Antibodies and Developments in Immunoassay, Elsevier North Holland Biomedical Press, Amsterdam (1981) 3. [Pg.166]