Alternative Chromatographic Modes and Techniques

In consideration of the above it is therefore essential to ecamine, as widely as possible within the time available, all of the alternatives which are available to the chromatographer, in order to develop the required safe, reliable and cost-effective process. Throughput must be maximised in order to minimise the use of often flammable, toxic and costly solvents and to minimise expenditure on plant, equipment, labour and operating costs. 

Tiselius [3] defined three distinct modes in which a chromatographic column can be operated, elution, displacement and frontal. In addition to this there are ways of operating chromatographic columns which can be considered as intermediate between these modes and, in addition, there are various techniques which have been applied to chromatographic separations in order to improve throughput. These will be discussed in this Section. 

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