Alpha Innotech

Alpha Innotech NovaRay CCD camera with a broadband excitation source with up to eight excitation and emission filters for imaging slides or microtiter plates. www.alphainnotech.com... 

Standard UV transilluminator or a laser scanner (HuorChem 8800, Alpha Innotech Imager, San Leandro, CA, USA) see Note 4). 

Expose the membranes using either Hyperfilm ECL or a chemiluminescence imaging system, such as the FluorChem imager (Alpha Innotech Corp.). In either case, try several different exposures of varying lengths of time (e.g., 30 s-5 min) see Fig. 3). 

The blots are washed with TEST again three times for 15 min each and then developed with SuperSignal West Femto Maximum Sensitivity Substrate. Images are captured with a CCD camera such as the FluorChem 9900 Imaging System from Alpha Innotech. 

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