Alkylamino acids specifications

The separation of nucleic acids, particularly t-RNA, has been another useful application of HIC for biological macromolecules. The tertiary structure of t-RNA has made analysis under the gentle conditions of HIC very feasible [12]. Figure 2 shows an example of the purification of t-RNA molecules specific for different amino acids on a lOO-nm polyol HIC column. Separation of t-RNA molecules has also been accomplished successfully by using HIC conditions on supports with alkylamino ligands, which are functionally similar to those traditionally used to separate nucleic acids [I]. 

In addition to the classical form of the Michaelis-Arbuzov reaction, several non-classical variations have proved to be of great use with regard to general applicability and for the preparation of specific compounds. For instance, the ring opening of A-acylaziridines, by either trialkyl phosphites or phosphonous diesters, or by tris(trimethylsilyl)phos-phite, leads to dialkyl [2-(A-alkylamino)ethyl]phosphonates, or their bis(trimethylsilyl) diesters in A-protected forms from which A-protection may be removed under aqueous acid conditions (Scheme 13). 

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