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Alkaline phosphatase chemical modification

Nucleic acids can also be biotinylated by nonenzymatic methods with photobiotin, a photoactivatable biotin analog (6), which can be commercially obtained from BRL, Sigma, and other commercial sources I have not compared the suitability of this method of biotin incorporation with that reported here, but expect that the method would be fully acceptable FMC (Rockland, ME) markets an alternate nonradioactive sequence detection kit known as Chemiprobe. The basis of this system is a chemical modification of cytosine residues m the probe DNA. After hybridization, the probe is detected by means of a monoclonal antibody that specifically recognizes the sulfonated DNA. Detection of the bound monoclonal antibody is achieved by means of an alkaline phosphatase-conjugated second antibody. [Pg.403]

Amperometric biosensor based on carbon fiber microelectrodes, modification with alkaline phosphatase, 238-239 Anion chemical sensing, pyrophosphate, 315-317,318 319/... [Pg.326]

See other pages where Alkaline phosphatase chemical modification is mentioned: [Pg.2502]    [Pg.21]    [Pg.139]    [Pg.41]    [Pg.205]    [Pg.53]    [Pg.211]    [Pg.163]    [Pg.2502]    [Pg.21]    [Pg.228]    [Pg.102]   
See also in sourсe #XX -- [ Pg.389 , Pg.390 , Pg.391 , Pg.427 ]

See also in sourсe #XX -- [ Pg.391 , Pg.427 ]

See also in sourсe #XX -- [ Pg.391 , Pg.427 ]



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