Alginates Assay

Assay Determine as directed under Alginates Assay, Appendix IIIC. Each milliliter of 0.25 A sodium hydroxide consumed in the assay is equivalent to 25 mg of (CgHgOg), (equiv wt 200.00). 

Assay Determine as directed under Alginates Assay, Appendix me. 

Algin, 24, 54,312, 351 Alginates Assay, 768 Algin Derivative, 331 AlginicAcid, 19 Alkaline Borate Buffer, 848 Alkaline Cupric Citrate TS, 851 Alkaline Cupric Tartrate TS, 849 Alkaline Mercuric-Potassium Iodide TS, 850... 

Quantitative spectrophotometric methods for pectin utilize carbazole (diphenyleneimine Bitter and Muir, 1962) and m-hydroxydiphenyl (Kintner and Van Buren, 1982). The intense red-to-brown color with carbazole in sulfuric acid, relatively specific for uronans (pectin and alginate), is much less intense with ketohexoses, aldohexoses, and pentoses (Snell and Snell, 1953). The m-hydroxydiphenyl assay is subject to less interference than the carbazole assay. 

Fig. 4. H2 photoevolution from ascorbate via P. laminosum PSI particles under different conditions of immobilization. The complete assay system contained 100 mM Mes-NaOH buffer, pH 7.0 75 mM sodium ascorbate 15 mM DTT 2 mM TMPD 1% (w/v) BSA PSI particles (30 pg Chi) 50 pi (saturating amount) of Clostridium pasteurianum hydrogenase and 12.5 pM Spirulina maxima Fd as electron mediator, (a) Conditions all components free (b) hydrogenase immobilized in Ca alginate according to Gisby and Hall (1980) (c) PSI particles immobilized in Ca alginate (d) hydrogenase and PSI coimmobilized in Ca alginate.

Assay A sample yields not less than 18% and not more than 21% of carbon dioxide (CO2), corresponding to between 88.7% and 103.6% of Ammonium Alginate (equiv wt 217.00), calculated on the dried basis. 

Wainwright M. (1981) Assay and properties of alginate lyase and 1.3-(3-glucanase in intertidal sands. Plant Soil 59, 83-89. 

C, com starch W, water S, sucrose X, xanthan gum G, guar gum A, alginate. Standard error (SE) between parentheses. Assays were performed in quintuplicate. Within a column, values with different superscripts are significantly different (P <. 05). 

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