Aleurone layer, analysis

Ogawa, M., Tanaka, K., and Kasai, Z., 1979, Accumulation of phosphorus, magnesium, and potassium in developing rice grains Followed by electron microprobe X-ray analysis focusing on the aleurone layer. Plant Cell Physiol. 20 19-27. 

Microspectrofluorometry was employed for mapping the location of phenolic substances in maize kernels. Autofluorescence due to phenolic acids was detected mainly in the embryo, aleurone and pericarp of maize kernel cross sections. Boric acid (H3BO3) reagent enhanced the fluorescence due to flavonoids in the aleurone layer. The amides of phenolic acids required derivatization with Ehrlich s reagent (168) to reveal fluorescence in the embryo and aleurone. The localization of phenolic amines was conflrmed by HPLC analysis. Phenolic compounds are important in the resistance of maize kernels to pests. Resistant maize types showed higher intensities of phenolic fluorescence but no unusual distributions of these compounds. ... 

Fig. 3. Effect of the transcription inhibitor, cordycepin, on the action of ABA in barley aleurone layers. Northern blot analysis is the same as described under Fig. 2. Aleurone layers were incubated for 25 h in the absence of GA3 (lane 7), 25 h in the presence of lO M GA, (lane 2), 24 h in the presence of GA, and 2 X lO M ABA with a second addition of ABA at 12 h (lane i), 25 h in the presence of GA3 with ABA added 10 h after GA3 (lane 4), 25 h in the presence of GA3 with ABA added 20 h after GA3 (lane 5), or 25 h in the presence of GA3 with ABA and 10 " M cordycepin added 20 h after GA3 (lane 6). Note that cordycepin addition prevented the reduction of a-amylase mRNA induced by ABA (cf lanes 5 and 6). From Nolan et al. [19]...

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