Aldosterone, radioactive

This step proceeds through the hydroxy-7V-acetate (418), which undergoes rearrangement in acetic anhydride-sodium acetate to give the iV-acetyl-acetate (419). Further simple steps afforded 1,2-didehydroaldosterone 18,21-diacetate (420), permitting tritiation to produce radioactive-labelled aldosterone. Several oxidations of the nitrone (417) are also described Jones chromic acid in aqueous acetone... 

In contrast to most it-Cw, A5-C2i or estrogen conjugates, comparative studies with corticosterone and corticosterone-21-sulfate injected into the umbilical vein establish that this ester sulfate is transferred to the mother slightly more quickly than the free hormone (see Section III, D, 3 and Table IV). These studies also demonstrate that the transfer rates of the free corticosteroids from the fetus to the mother are very different after perfusion of corticosterone, cortisol, cortisone, and deoxycortisosterone into the umbilical vein at midgestation, these steroids were transferred very slowly (2-8% of the administered radioactivity is collected in the maternal urine) on the other hand, after tritiated aldosterone was perfused through the umbilical vein, 20 to 30% of the radioactivity was recovered in the maternal urine (Pasqualini, 1970). 

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