Aldosterone glucuronide

Hot acid hydrolysis, the first method used, produced many artifacts and was generally abandoned. However, for labile glucuronides such as aldosterone glucuronide, where the C-18 hemiacetal is linked to glucuronic acid, hydrolysis at pH 1 at room temperature is a method of choice (Underwood and Tait, 1964). 

The metabolism of aldosterone is similar to that of cortisol, about 50 mcg/24 h appearing in the urine as conjugated tetrahydroaldosterone. Approximately 5-15 mcg/24 h is excreted free or as the 3-oxo glucuronide. 

UGT2B15 Human Liver, prostate, testes, adipose tissue Steroids UGT2B17 glucuronidates preferentially at the 17-OH positions of androgens (>30H) Aldosterone, 5a- and 5 3-dihydroaldosterone,... 

Aldosterone and cortisol circulate in protein-bound states and have different fractional distributions (see Table 30-3). Because of the relatively large fraction of unbound (40%) and loosely bound (50%) forms, aldosterone is cleared from blood much faster than is cortisol (half-lives in blood aldosterone, 30 minutes cortisol, 10 hours). Both steroids are metabolized mainly in the liver. The major metabolite of aldosterone is tetrahy-droaldosterone glucuronide and that of cortisol is tetrahy-drocortisol glucuronide. These water-soluble conjugates are excreted by the kidney. Another metabolite of cortisol is cortisone (4-pregnen-17Q ,21-diol-3,l 1-20-trione), which has no intrinsic activity but can be reconverted to cortisol in certain peripheral tissues (e.g., lung, liver). 

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