Aldolase gene, mutations

T16. Tolan, D. R Molecular basis of hereditary fructose intolerance Mutations and polymorphisms in the human aldolase B gene. Hum. Mutat. 6,210-218 (1995). 

Hereditary fructose intolerance is a rare recessive genetic disease that is most commonly caused by a mutation in exon 5 of the aldolase B gene. The mutation fortuitously creates a new Ahall recognition sequence. To test for the mutation, DNA was extracted from a wife, husband, and their two children. Jack and Jill. The DNA for exon 5 of the aldolase B gene was amplified by polymerase chain reaction (PCR), treated with Ahall, subjected to electrophoresis on an agarose gel, and stained with a dye that binds to DNA. 

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