AKTA explorer™ FPLC

AKTA explorer FPLC system with a UV/Vis 920 module, operated with Unicorn software suite (GE Healthcare, Uppsala, Sweden). 

When the automatic AKTA explorer FPLC system is used, after the sample loading, wash the columns with the equilibration buffer until the absorbance of the collected fractions at 280 nm is re-zeroed (reaches a value <0.005). 

Fluorescent chemokine fusion proteins are isolated using an automated chromatography system (AKTA FPLC or explorer or similar). Load culture supernatants (50-150 mL) on a 5 mL FhTrap Flep-arinFlP column equilibrated with buffer A (10 mMNaPi,pH 7.3) at a flow rate of 3 mL/min (Fig. IB). 

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