Agrobacterium tumefaciens enzym

A novel decarboxylase, 2,6-dihydroxybenzoate decarboxylase, was found in Agrobacterium tumefaciens 1AM 12048 at first. Thereafter, the same activity was found in Rhizobium species by two groups independently. Furthermore, Pandoraea sp. 12B-2, the most powerful producer of 2,6-dihydroxybenzoate decarboxylase, was isolated. These enzymes have been purified and characterized. 

Human growth hormone was expressed as fusion with the Agrobacterium tumefaciens nopaline synthase enzyme but only transcript was detectable... 

The enzymes placed in class IV are from bacteria mainly utilizing the Entner—Doudoroff pathway, and the ADP-Glc PPases are distinctively activated by Fru 6-P and pyruvate, with ADP, AMP, and P behaving as inhibitors. " Included in this group are Agrobacterium tumefaciens and Arthrobacter viscosus. Photosynthetic... 

Akiyoshi, D.E., Klee, H., Amasino, R.M., Nester, E.W. Gordon, M.P. (1984). T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proceedings of the National Academy of Science USA, 81 5994-5998... 

Aloni Y, Delmer D.P., and Benziman M. 1982. Achievement of high rates of in vitro synthesis of 1, 4-beta-D-glucan activation by cooperative interaction of the Acetobacter xylinum enzyme system with GTP, polyethylene glycol, and a protein factor. Proc Natl Acad Sci USA 79(21) 6448-6452. Amikam D. and Benziman M. 1989. Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens. J Bacterid 171(12) 6649-6655. 

This chapter describes the characterization of a dihydropyrimidinase from Sinorhizobium meliloti (SmelDhp), and provides an in-depth study of the prokaryotic NCpAA isolated from Agrobacterium tumefaciens (Atpcar). It goes on to explain how the combination of both enzymes converts DHU derivatives into P-amino acid by a chemo-enzymatic cascade. 

S., and Las Heras-Vazquez, F.J. (2011) N-carbamoyl-P-alanine amidohydrolase from Agrobacterium tumefaciens C58 a promiscuous enzyme for the production of amino acids. J. Chromatogr. B Anal. Technd. Biomed. Life Sci., 879 (29), 3277-3282. 

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