AFM studies of biochemically manipulated or reconstituted chromatin fibers

AFM studies of biochemically manipulated or reconstituted chromatin fibers 

As already noted above, AFM imaging and quantitations on LH-stripped chromatin fibers revealed extended beads-on-a-string structures, in agreement with early EM results [17]. Nucleosomal arrays reconstituted from naked DNA fragments and histone octamers had similar appearance [36 2]. 

The contribution of individual core histone N-tails to transcriptional regulation has been recently addressed in a reconstitution study [42]. Varied combinations of recombinant and mutant cores histones have been assembled on circular plasmids, and the resultant nucleosomal arrays were tested for transcriptional 

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