Affinity precipitation from partially

Affinity Precipitation of Avidin from Partially Purified Egg Whites. Following the ion exchange fractionation of egg white solution, described in the experimental section above, fractions of eluted protein solution (obtained from the elution described in Figure 6) were mixed with... 

The use of a novel affinity precipitation scheme, applicable to multibinding site biomolecules, has been illustrated by the purification of avidin from model protein solutions and from partially purified egg whites. The... 

Figure ll. SDS-PAGE patterns from (a) standard Vector Labs avidin solution, (b) Fraction B from ion-exchanged partially purified egg whites before affinity precipitation (described in experimental section), (c) same as (b) but after affinity precipitation, and (d) purified avidin from Fraction B after affinity precipitation and reconstitution. 

Proteinase A was purified from yeast extracts, activated by incubation at pH 5, by repeated chromatography on DEAE-Sephadex followed by alcohol precipitation, as described by Hata et al. (3). The enzyme could be further purified in our laboratory by affinity chromatography of the partially purified enzyme, using the specific proteinase A-inhibitor I, bound to activated CH-Sepharose 4B as the stationary phase. The pattern of elution by 6 M urea from the affinity column is shown in Figure 1 the peaks of hemoglobinhydrolyzing activity (i.e. proteinase A activity) and protein concentration coincide. Table II summarizes the present purification procedure for proteinase A. Work on further purification and characterization of the enzyme is in progress. 

The enzymatic synthesis of [ C]18 1-ACP Isoforms was according to a published procedure (9), and was purified through the octyl sepharose step. Oleoyl-ACP thloesterase was partially purified from spinach tissue by methods which Involved acid precipitation and either Ion-exchange or affinity chromatography (7,8). Glycerol-3-phosphate acyl transferase was purified by ammonium sulfate fractionation, DEAE and... 

Abietadiene synthase, an enzyme which catalyzes the cyclization of geranylgeranyl pyrophosphate to 7,13-abietadiene. (fig. 1) has been partially purified from the lOO.OOOg supernatant of young needle extract by a combination of PEG precipitation, ion exchange, affinity color and gel filtration chromatographies. 

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