Affinity Determination Between hIL8 and Its Antibody Fragments

Affinity Determination Between hIL8 and Its Antibody Fragments 

streptavidin was bound to the biotin SAM. Typically, the density of the SAM was 2-2.5ng mm 2. Next, the biotin-Fab was attached. The saturation density of biotin-Fab was 2.7—2.9 ng mm-2. In order to reduce the influence of steric hindrance/mass-transport/rebinding, a moderately reduced Fab density level was used, i.e., 2.1 ng mm-2 for Fabl3A and 1.7ng 

The association/dissociation curves are shown in Fig. 12(B). A common pseudo first-order kinetic model was applied to yield the kinetic constants (kon and ko ) and the affinity constants (KA and A D). The association curves could 

Surface Plasmon Optics for the Characterization of Biofunctional Architectures 

The affinity constant KA and dissociation constant KD can then be calculated according to 

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