Affinity chromatography immunoaffinity chromatograph

Chapter 15 provides an in-depth coverage of various chromatographic methods, such as ion exchange, hydrophobic interaction, affinity, ligand, immunoaffinity, gel filtration, etc., that can be used for separations of antibodies by liquid chromatography. 

The fundamentals of affinity chromatography can be taken from the literature [2-5]. Since affinity-based analytical methods are extremely dependent on the analytes and their respective binding molecules, it is not possible to go into much detail with regard to practical advice. It has to be mentioned that affinity chromatography is rarely a true chromatographic technique, and in most cases has to be considered to be a selective extraction method. Hence, the term affinity extraction or immunoaffinity extraction should be preferred where appropriate. 

Zolotarjova N, Boyes B, Martosella J. Immunoaffinity depletion of high-abundant proteins for proteomic sample preparation. In Smqkal GB, Lazarev A, editors. Separation methods in proteomics. Boca Raton, FL CRC Press 2006. p. 63—79. Moser AC, AC, Hage DS, DS. Chromatographic immunoassays. In Hage DS, editor. Handbook of affinity chromatography. 2nd ed. Boca Raton, FL CRC Press 2005. p. 789-836. 

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