Affinity chromatography diagram

An example of a successful application of affinity chromatography is the isolation of the enzyme cytidine deaminase from cells of E. coli. Cytidine was linked covalently via long spacer arms to the agarose beads as in the following diagram ... 

Fig. 17.10. Schematic diagram of the ICAT strategy to proteomics. Samples are labelled, pooled and digested and undergo ICAT affinity chromatography to select cysteine-labelled peptides. Relative quantifications between proteins from both sample states are gained and tandem MS is performed to provide an identification.

Fig. 3. Affinity chromatography, (a) Schematic diagram of affinity chromatography (b) elution diagram indicating that nonspecific proteins that do not bind to the immobilized ligand pass straight through the column, while the specific protein binds to the immobilized ligand and is eluted from the column only on addition of soluble ligand.

Fig. 36.—Anti-glucoamylase antibodies. A Diagram of segment of the structure of gluco-amylase. B Affinity chromatography of glucoamylase immune serum. Anti-glucoamylase antibodies (Ab), diffused against glucoamylase (G) and periodate-oxidized glucoamylase (xG). [From J. H. Pazur, K. R. Forry, Y. Tominaga, and E. M. Ball, Biochem. Biophys. Res. Commim., 100 (1981) 420-426.]...

Figure 2 Cause-and-effect (fish-bone) diagram for affinity chromatography purification.

Fig. 3a. Flow diagram for the separation of the components of a complex mixture of oligosaccharides by serial lectin affinity chromatography. Depending upon the lectin adsorbant, specific oligosaccharides are either unbound (not retarded by the adsorbant), retarded (and eluted without the need of a saccharide inhibitor), or are tightly bound and then require either lOmM methyl a-D-glucopyranoside or lOOmM methyl a-D-mannopyranoside for elution. Where appropriate, each eluted peak is concentrated and the saccharide inhibitor is removed prior to application to the second affinity column. The structures of the individual oligosaccharides are shown in Fig. 3b. (Adapted from ref 288.)...

Fig.1, Ugl reaction product and scaffold formation on solid-support, (a) The Ugi reaction comprises an oxo-component (aldehyde or ketone) (R1), a primary or secondary amine (R2), an isonitrile group (R3), and a carboxylic acid (R4), which are condensed to yield a single product (1) In a "one-pot" reaction, (b) The proposed solid-phase Ugi reaction comprises an aldehyde-tunctionalized matrix to which the other three solution-phase components are added to yield a single ligand scaffold (2) (reproduced from (17) with permission trom Elsevier), (c) Putative solid-phase Ugi reaction mechanism for affinity chromatography ligand formation on aldehyde-tunctionalized chromatography sorbent. Tbe diagram shows only one ligand per bead tor clarity. (Reproduced trom (16) with permission from the author).

Figure 1 Schematic diagram of some of the possible interaction patterns in affinity CE with hypothetical, homogeneous receptor-ligand systems characterized by different reaction kinetics. Ref. is a noninteracting component, whereas the other peak represents a molecule interacting quickly (second panel from top) or more slowly (lower panels) with ligands of lesser electrophoretic mobility, which are present in the electrophoresis buffer (42). (Reproduced with permission from the copyright holder, Elsevier Science Publishers and Journal of Chromatography. )...

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