Affinity chromatography dextran gels

Classical LLPC using aqueous-aqueous polymer systems based on Albertsson s [9] PEG-dextran system has provided a versatile tool for the separation of proteins and nucleic acids, thus increasing the arsenal of biopolymer purification methods currently dominated by gel filtration, ion-exchange chromatography, and affinity chromatography RPC. The technique operates... 

The very same principle was applied by Suzuki et al. [19] for the purification of the placental insulin receptor, starting with a prepurified sample, obtained from a plasma membrane fraction of human placenta by a fractionation protocol including affinity chromatography on Sepharose-concanavalin A as the last step. The following purification step was carried out, using insulin bound to dextran (M 40000) as the biospecific, water-soluble carrier. After filtration of the dextran-insulin-receptor complex on an adequate gel and then dissociation by mild acidification, the insulin receptor was obtained in a purified form. This method permitted a 8700 fold purification, from crude plasma membrane and, even more remarkable, a 60-fold purification after the affinity chromatography step. 

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