Affinity chromatography batch process

Arve and Liapis [34] suggest estimating the parameters characterizing the intraparticle diffusion and the adsorption-desorption step mechanisms of affinity chromatography from the experimental data obtained in a batch system. The numerical simulations of the chromatographic process will use the values of the parameters of the adsorption isotherm and those of the effective pore diffusion as determined from stirred tank experiments together with the film mass transfer coefficients calculated from chemical engineering expressions found in the literature. 

In practice, affinity chromatography is usually designed to be the final (if not the sole) chromatographic step in a purification procedure, so as to protect the valuable affinity material from unnecessary contamination. It is normal to use short columns whose capacity is largely fully exploited. Elution can be by a batch process or gradi-... 

---