Adsorbent and Phase Systems

Find a mobile phase with high solubility, a compatible stationary phase, and then optimize selectivity, capacity, and efficiency followed by a systematic increase in column load. 

This is certainly true for the technical and production scale domain, but for high-throughput separations or other projects, where time dominates productivity, other solutions have to be applied based on limited or even not optimized methods. 

These two main fields of application for chromatography result of course in a dissimilar approach to selecting the chromatographic system. If time pressure 

For production-scale processes, economic pressure arises and thus a successive optimization of the chromatographic system will pay offby a reduction in operating costs. Therefore, the development of the chromatographic system has to start with the search for a suitable mobile phase with high solubility for the given solute (Section 3.2.3.1) and a compatible adsorbent. Dependent on the nature of the phase system the separation is optimized by adjusting the mobile phase composition (Sections 3.2.4—3.2.6). 

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