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Adipic acid dihydrazide phosphates

Dissolve 32mg of adipic acid dihydrazide per ml of 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2. [Pg.142]

Figure 1.112 Phosphate groups can be modified with adipic acid dihydrazide in the presence of a carbodi-imide to produce hydrazide derivatives. This is a common modification route for the 5 -phosphate group of oligonucleotides. Figure 1.112 Phosphate groups can be modified with adipic acid dihydrazide in the presence of a carbodi-imide to produce hydrazide derivatives. This is a common modification route for the 5 -phosphate group of oligonucleotides.
Add to the tube 7.5 pi of RNA or DNA containing a 5 -phosphate group. The concentration of the oligonucleotide should be 7.5-15 nmol or total of about 57-115.5 pg. Also, immediately add 5 pi of 0.25 M adipic acid dihydrazide or carbohydrazide dissolved in 0.1 M imidazole, pH 6.0. Because EDC is labile in aqueous solutions, the addition of the oligo and fezs-hydrazide/imidazole solutions should occur quickly. [Pg.146]

Dissolve 160 mg of adipic acid dihydrazide (Aldrich) in 5 ml of 0.1 M sodium phosphate, pH 6.0. Some heating of the tube under a hot-water tap may be required to help solubilize the compound. Cool to room temperature. [Pg.919]

To make an amine derivative of dextran, dissolve ethylene diamine (or another suitable diamine) in 0.1 M sodium phosphate, 0.15 M NaCl, pH 7.2, at a concentration of 3 M. Note Use of the hydrochloride form of ethylene diamine is more convenient, since it avoids having to adjust the pH of the highly alkaline free-base form of the molecule. Alternatively, to prepare a hydrazide-dextran derivative, dissolve adipic acid dihydrazide (Chapter 4, Section 8.1) in the coupling buffer at a concentration of 30 mg/ml (heating under a hot water tap may be necessary to completely dissolve the hydrazide compound). Adjust the pH to 7.2 with HC1 and cool to room temperature. [Pg.956]

The following protocol describes the modification of DNA or RNA probes at their 5 -phosphate ends with a bis-hydrazide compound, such as adipic acid dihydrazide or carbohydrazide. A similar procedure for coupling the diamine compound cystamine can be found in Section 2.2 (this chapter). [Pg.980]

Adipoyldiamino) bis [2,4,6-triiodobenzoic acid] 3,3 -(Adipoyldiimino) bis (2,4,6-triiodobenzoic acid). See lodipamide Adipoyl hydrazide. See Adipic acid dihydrazide Adi-pure . See Adipic acid Adipyinitrile. See Adiponitrile Adjunct B. See Sodium phosphate dibasic anhydrous... [Pg.123]


See other pages where Adipic acid dihydrazide phosphates is mentioned: [Pg.146]    [Pg.143]    [Pg.144]    [Pg.563]    [Pg.123]    [Pg.124]    [Pg.50]   
See also in sourсe #XX -- [ Pg.146 ]

See also in sourсe #XX -- [ Pg.124 ]

See also in sourсe #XX -- [ Pg.124 ]




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ADIPATE

Acidic phosphates

Adipic acid

Adipic acid dihydrazide

DIHYDRAZIDE

Dihydrazides

Phosphate acid

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