Adenylylation of Small GTPases

Addressing these basic questions requires analytical approaches that combine enzyme biochemistry, biophysics, and chemical synthesis. In this chapter we present one possible route to analyze new PTMs exemplified by the adenylylation of Rabl. 

The analysis of adenylylation requires preparatively adenylylated proteins in order to determine the site of the PTM, to determine the structure of the modified proteins, and to establish the functional consequences for Rabl-activity. Also, the systematic investigation of adenylylation in live cells and their distribution among different prokaryotic and eukaryotic proteomes requires specific tools 

In this subchapter we therefore describe the preparation of adenylylated proteins and peptides for in vitro studies. 

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