Adenosine 5‘- , ADPaS

It is a well established property that many enzymes interact in a stereospecific manner with the different diastereoisomers of nucleoside phosphorothioates. For example, when AMP is substituted by adenosine S -t7-monopho phorothioate (AMPS), adenylate kinase-catalysed phosphorylation occurs specifically at the pro-R oxygen atom to give (5ip)-ADPaS. The stereospecificity results from the restricted orientation of the P-O and P-S bonds in the enzyme active site. While it is known that changing the metal ion can perturb the stereoselectivity, Tsai and... 

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