Activating point mutations

Bhattacharya A, Dang H, Zhu QM, et al. Uropathic observations in mice expressing a constitutively active point mutation in the 5-HT3A receptor subunit. J Neurosci 2004 24(24) 5537-5548. 

Ramakrishnan B, Qasba PK. Structure-based design of pi,4-galactosyltransferase I (f 4Gal-Tl) with equally efficient N-acetylgalactosaminyltransferase activity point mutation broadens P4 gal-Tl donor specificity. J. Biol. Chem. 2002 277 20833-20839. 

Figure 17.14 Model of evolved mutant from cephalosphorinase shuffling. The sequence of the most active cephalosporinase mutant was modeled using the crystal structure of the class C cephalosporinase from Enterobacter cloacae. The mutant and wild-type proteins were 63% identical. This chimeric protein contained portions from three of the starting genes, including Enterobacter (blue), Klebsiella (yellow), and Citrobacter (green), as well as 33 point mutations (red). (Courtesy of A. Crameri.)...

Burger M, Burger JA, Hoch RC, Oades Z, Takamori H, Schraufstatter IU. Point mutation causing constitutive signaling of CXCR2 leads to transforming activity similar to Kaposi s sarcoma herpesvirus-G protein-coupled receptor. 1 Immunol 1999 163(4) 2017-2022. 

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