Actinomycins biosynthesis, amino acid synthesis

Adipose tissue obtained from diabetic animals shows a decrease of the incorporation of labeled amino acid into protein and in the activity of a number of enzymes hexokinase II, glucose-6-P04 dehydrogenase, phosphofructokinase and pyruvate kinase. Amino acid incorporation and enzyme activities return to normal after injection of insulin. The effect of insulin is, at least in the case of hexokinase II and glucose-6-PO4 dehydrogenase, impaired by the injection of actinomycin D indicating that insulin stimulates de novo protein synthesis by triggering the biosynthesis of new messenger RNA. 

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