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Acid glycosaminoglycans, quantitation

Infrared spectra of glycosaminoglycans in D20 and DC1 were used165 for quantitative evaluation of the uronic acid and acetamido groups in this class of polysaccharide. The results showed that i.r. spectroscopy of compounds in D20 solution provides a simple, quantitative basis for examination of ras(COJ) and amide I bands in heparin and chondroitin 4-sulfate. In DC1, analysis was based on p(C02H) and amide I bands which were well resolved. The apparent acid-dissociation constants of the studied polysaccharides were estimated from the absorbance of p(C02) or p(C02H) bands, or both, at different pH (pD) values. [Pg.55]

Biological Applications Glycoconjugates assay hyaluronic acid assay hybridization assay cell agglutio-nation detecting humic acid, detecting pancreatic B-granules locomotion of amoeba, " quantitative deter-mination of glycosaminoglycans, ... [Pg.8]

See other pages where Acid glycosaminoglycans, quantitation is mentioned: [Pg.258]    [Pg.146]    [Pg.327]    [Pg.14]    [Pg.382]    [Pg.23]    [Pg.50]    [Pg.62]    [Pg.67]    [Pg.326]    [Pg.208]    [Pg.290]   


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