Acetylkanamycin amidohydrolase

N-acetylkanamycin amidohydrolase in the idiopha-se of kanamycin fermentation, Aqr. Biol. Chem. 

Satoh A, Ogawa H, Satomura Y. Regulation of N-acetylkanamycin amidohydrolase in the iJiophase in kanamyctn fermentation. Agric Biol Chem 1976 40 191-196. 

In most cases the mechanism by which nutrients control secondary metabolism is unknown. However, glucose and other rapidly used carbon sources repress the formation of iV-acetylkanamycin amidohydrolase, thought to be the final enzyme of kanamycin A biosynthesis (D 1.3) and phenoxazinone synthase, an enzyme required in actinomycin biosynthesis (D 8.4.1). 

The report that cAMP relieves glucose repression of iV-acetylkanamycin amidohydrolase in Streptomyces kanamyceticus, a prokaryote ( ), indicates that the repression mechanism resembles that of different catabolic enzymes in bacteria, which proceed via the inhibition of adenylate cyclase, the enzyme that converts ATP to cAMP (D 10.4). As a consequence the concentration of cAMP decreases and the transcription by RNA polymerase of operons subjected to cAMP control is inhibited (catabolite repression). In eucaryotes, however, catabolite repression could not be demonstrated. In Penicillium cyclopium for instance, glucose suppression of benzodiazepine alkaloid biosynthesis cannot be overcome by administration of cAMP or cAMP derivatives. 

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