Acetylcholine redox potential

The primary objective of ChE-ChOx bienzyme biosensors was to determine neurotransmitter acetylcholine in biological samples (Chen et al, 1998). The redox potential of acetylcholine (ACh) is too high to be determined directly using electrochemical reaction. For this purpose, acetylcholine esterase (AChE) was employed as ChE. AChE and ChOx were immobilized on the surface of the electrode to attain electrochemical analysis according to reactions (4) and (5) (Scheme 55.3). 

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