2-Acetamido-2-deoxy-D-glucopyranosides

Fig. 6. Elution profiles of three alkyl 2-acetamido-2-deoxy- -D-glucopyranosides 4a (a. n=8 b. n=ll c. n=14) in gel filtration chromatography on sepharose 4B (exclusion 2.10 Da, exclusion volume 10 mL) monitored by and radioactivity countings (normed to 1.0 for reason of convenience). ( ) H countings of H-labelled phosphatidyl choline (x) C...

Substrate/buffer solution dissolve 20 mg 4-methylumbelliferyl-2-acetamido-2-deoxy-/>-D-glucopyranoside (MW 379.4) in 10.5 ml of reaction buffer to a final concentration of 5 mM. 

Chitin, or poly (l,4 -0-j -2-acetamido-2-deoxy-D-glucopyranoside), a j3-l,4-linked polymer ofN-acetylglucosamine... 

Methoxy-2-pyridyl 2-acetamido-2-deoxy- -D-glucopyranoside (i3) as a Glycosyl Donor... 

Characteristic differences in the n.m.r. chemical shifts of the mono- and di-O-methyl derivatives of methyl 2-acetamido-2-deoxy-D-glucopyranoside have been observed and should be of value in the structural analysis of carbohydrate chains containing these residues. ... 

The Km values and relative catalytic constants have been determined for the bovine liver jS-D-2-acetamido-2-deoxyglucosidase-catalysed hydrolysis of a series of substituted-phenyl 2-acetamido-2-deoxy- -D-glucopyranosides. Plots of log Km with respect to Hammett substituent constant and Hantsch substituent constant showed the enzyme-substrate affinity to be dependent on the electronic nature of the substituents but not on the hydrophobic nature. Methanol competed with water for the glycosyl-enzyme to some extent, but did not increase indicating that the deglycosylation is not rate limiting. 

The interactions of the substrate analogues methyl 2-acetamido-2-deoxy- -D-glucopyranoside, di-iV-acetylchitobiose, and tri-A -acetylchitotriose with hen egg-white lysozyme in which an ester linkage had been formed between L-glutamic acid 35 and L-tryptophan 108 were studied by c.d. and fluorescence techniques and were compared with those for the native enzyme. The binding... 

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